Original research article
GC/MS Based Metabolite Profiling and Antioxidant Activity of Balkan and Bulgarian Endemic Plants
2019, 84 (1) p. 59-65
Milena Nikolova, Ina Aneva, Petar Zhelev, Strahil Berkov
Abstract
Balkan and Bulgarian endemic plants Viola rhodopaea Becker (Violaceae), Veronica rhodopaea (Velen.) Degen ex Stoj. & Stefanov (Plantaginaceae), Silene roemeri Friv. (Caryophyllaceae), Jasione bulgarica Stoj. & Stef and Campanula lanata Friv. (Campanulaceae) were examined. Metabolite profiles of methanolic extracts of studied species were analyzed by GC/MS and HPTLC. Total flavonoid and phenol contents were determined by aluminum chloride (AlCl3) and Folin-Ciocalteu’s reagent, respectively. Antioxidant potential of the extracts was assayed by DPPH test. Fatty acids and alcohols, phenolic and organic acids, flavonoid aglycones and glycosides, sterols and carbohydrates were identified. Jasione bulgarica and Viola rhodopaea extracts were determined to have the highest flavonoid and phenol content. Significant radical scavenging activity was estimated for the extracts of J. bulgarica, Veronica rhodopaea and Viola rhodopaea. The present information of chemical composition and antiradical potential of studied species are reported for the first time.
Keywords
Viola rhodopaea, Jasione bulgarica, Campanula lanata, Veronica rhodopaea, Silene roemeri
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