Original research article
Micropropagation of Grape Cultivars (Vitis vinifera L.) on Different Basal Media Supplemented with Benzyl Adenine
2016, 81 (3) p. 123-129
Ali-Akbar Mozafari, Ommar Ghoraishi, Nasser Ghaderi, Teimoor Javadi
Abstract
In vitro propagation is a commercial technique that is used for plant propagation around the world. Therefore, the development of in vitro techniques is a sure way for rapid propagation of many plant species. In this study, effect of Woody Plant Medium (WPM) and Murashighe and Skoog (MS) media supplemented with 0.0, 2.2, 4.4, 6.6 and 8.8 μM benzyladenine (BA) on regeneration of three grape cultivars ('Bidaneh Sefi d’, ‘Farkhi’ and ‘Khoshnav’) were investigated. In proliferation stage, length of shoots, number of shoots, number of leaves and the final status of explants were evaluated. In rooting stage, effect of indole-3-butyric acid (IBA) in three concentrations (0.0, 0.5 and 1.0 μM) on root initiation, number of rooted explants, root length and number of roots were evaluated. The results showed that the longest shoots were obtained in MS medium supplemented with 2.2 μM BA in the three cultivars, while most adequate shoots were observed in MS medium containing 4.4 μM BA in all studied cultivars. In rooting stage, the best results were obtained by 0.5 μM IBA. The results of this study showed that grape regeneration potential in vitro conditions depend to cultivar, culture medium and concentration of growth regulators
Keywords
grape, regeneration, basal medium, in vitro
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