Original research article
In Vitro Propagation of Eggplant through Meristem Culture
2008, 73 (3) p. 149-155
Shamina Akhtar Sharmin, Ahmad Humayan Kabir, Abul Mandal, Kanak Kanti Sarker, Mohammad Firoz Alam
Abstract
Meristem culture was done for developing an efficient protocol of production of eggplant clones. Shoot tips of 30-35 days old field grown eggplants were used for meristem isolation. Three cultivars viz. ‘Islampuri’, ‘Khatkhatia’ and ‘Katabegun’ were used in the present investigation as explants source. Surface sterilization of shoot tips was found to be the best in 0.1% HgCl2 solution for 3 minutes. For primary establishment of isolated apical meristem in MS liquid medium containing 2.0 mg l-1 BAP was found the best in cv Islampuri. BAP was also proved to be best for the primary establishment of isolated apical meristem in all the cultivars. Subsequent development of meristem derived shoot was achieved in MS semisolid medium containing either 2.0 mg l-1 BAP and 1.0 mg l-1 NAA or 1.0 mg l-1 BAP. For root development from meristem derived shoots, 1.0 mg l-1 IBA was found most responsive in cv. ‘Islampuri’ and ‘Khatkhatia’. Aft er transplantation, the in vitro plants showed normal growth.
Keywords
shoot-tip, acclimatization, Solanum melongena L.
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