Original research article
The Effect of IBA and Putrescine on Rhizogenesis and Biochemical Changes in Cuttings of Olea europaea L. cv. ‘Konservalia’
2024, 89 (2) p. 113-126
Esmaeil Khaleghi, Sara Alivapour
Abstract
This study aimed to investigate the effect of IBA at different concentrations (0, 2000 and 4000 ppm) and putrescine (0, 100 and 200 ppm) on rhizogenesis and the biochemical changes of leafy hardwood cuttings of Olea europaea L. This experiment was conducted as a factorial in a complete randomized block design with three replications. The result showed that rhizogenesis was the highest and callus induction was the lowest in the treatment with 4000 ppm IBA. Furthermore, co-treatment with IBA and putrescine increased rhizogenesis and callus induction two-times over their individual treatments. Moreover, biochemical changes of protein, carbohydrate (C), nitrogen (N) percentage, C/N ratio, peroxidase activity and polyphenol oxidase showed significantly different results in two parts of the leaf (bud) and stem bark of the cutting (root and callus). Overall, based on the results, it can be concluded that IBA application at 4000 ppm can be recommended as a suitable treatment for rhizogenesis of hard-to-root ‘Konservalia’ cultivar.
Keywords
carbohydrate, cutting, nitrogen, peroxidase, polyphenol oxidase, rhizogenesis
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