Original research article
Cellulase and Polygalacturonase Activity by Trichoderma viride
2022, 87 (1) p. 35-43
Anatolii ANATOLIIOVYCH PAVLENKO, Evgeniy Pavlovych Kopilov, Olena Volodymyrivna Nadkernychna, Hanna Viktorivna Tsekhmister, Anna Serhiivna Kyslynska
Abstract
In this study the ability of Trichoderma viride IMB F-100076 to synthesize cellulase and pectinase enzymes was investigated. Clear zones in agar medium with Na-CMC, cellobiose and pectin as carbon sources were identified at different pH values. It was found that the mentioned fungus had cleaved Na-CMC, cellobiose and pectin, forming clear zones. The optimal pH value for cellulase and pectinase synthesis amounted to 5.0. At this value the clear zone and radial growth rate of T. viride was the highest. The ability of T. viride IMB F-100076 to synthesize cellulase enzymes and polygalacturonase was quantitatively proven. The maximum cellulase activity in static culture was reported on day 15 of cultivation. The total cellulase activity was 0.88 U mL-1, exoglucanase – 0.76 U mL-1, endoglucanase – 1.25 U mL-1 and β-glucosidase – 1.27 U mL-1. The highest value of polygalacturonase activity was 0.59 ± 0.01 U g-1 IDS reported at day 6 of cultivation.
Keywords
Trichoderma viride, cellulase activity, endoglucanase, exoglucanase, β-glucosidase, pectinolytic enzymes, polygalacturonase
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