During several collecting expeditions conducted in the Eastern part of Macedonia, district Probistip, 27 wild meadow fescue (Festuca pratensis Huds.) accessions were collected on different natural stands, like meadows and pastures. Accessions were documented, characterized, evaluated and seeds stored at -18°C. One of the traits evaluated was seed crude protein content, which is a desired trait for forage mixture components. The crude protein contents varied from 8.63 % / 100g DM for accession MKD 01514 to 14.6 % / 100g DM for accession MKD01496. Accessions grown on dry stands exhibited SCP content less than 10, 0 % / 100g DM, therefore accessions MKD01498, MKD01508 and MKD01514 may be recommended for further use on a stands with a good-water supply. The rest of the populations collected were growing on semi-moist or moist meadows, with the SCP content ≥11.0 % / 100g DM, providing material with a high seed protein content to be included in meadow fescue breeding programs.