Agriculturae Conspectus Scientificus, Vol 86, No 1 (2021)

Assessment of Rapid Diagnostic Tools in the Nitrogen Status of Inoculated Pea Plants (Pisum sativum L.)


Pages: 21-26


The pea, (Pisum sativum L.) as other legume plants, has symbiotic relationship with bacteria from genus Rhizobum that are able to perform biological fixation of atmospheric nitrogen due to the fact that plants are able to meet their seasonal nitrogen requirements. Nitrogen content in pea plant after artificial inoculation with ten indigenous strains of R. leguminosarum previously isolated in Herzegovina soils, referent check R. leguminosarum 1001 and the control with no inoculant, was investigated. The field study was set up in RCBD with four replicates at Hodbina (Mostar, Bosnia and Herzegovina) in 2009. Nitrogen was analyzed based on ten whole plants per plot. The total nitrogen in aboveground plant parts and number of pods were measured at anthesis. The total nitrogen in grains was measured at technological maturity. Relative amount of chlorophyll (un-dimensioned units) content and nitrates (NO3-N ppm) with the use of rapid diagnostic tools as Chlorophyll meter and Cardy ion meter was observed three times, just before flowering, at anthesis and at technological maturity. R. leguminosarum strains had affected total nitrogen content in the aboveground part of the peas comparing to the control (uninoculated seeds) and referent strain 1001. The treatment was not significant for number of pods per pea plant but significant differences appeared for applied strains and control, based on Cardy-ion meter readings with values in range from 246 to 387 mg NO3-N kg-1. Measured Chlorophyll content in the leaf showed no significant difference with respect to the applied indigenous strains R. leguminosarum compared to the control where values ranged from 46.87 to the 49.57 units. Measurements with both devices showed significant differences in obtained values due to the vegetation period.


pea, inoculation, indigenous strains, Chlorophyll meter, Cardy -ion meter

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