In this study the ability of Trichoderma viride IMB F-100076 to synthesize cellulase and pectinase enzymes was investigated. Clear zones in agar medium with Na-CMC, cellobiose and pectin as carbon sources were identified at different pH values. It was found that the mentioned fungus had cleaved Na-CMC, cellobiose and pectin, forming clear zones. The optimal pH value for cellulase and pectinase synthesis amounted to 5.0. At this value the clear zone and radial growth rate of T. viride was the highest. The ability of T. viride IMB F-100076 to synthesize cellulase enzymes and polygalacturonase was quantitatively proven. The maximum cellulase activity in static culture was reported on day 15 of cultivation. The total cellulase activity was 0.88 U mL-1, exoglucanase – 0.76 U mL-1, endoglucanase – 1.25 U mL-1 and β-glucosidase – 1.27 U mL-1. The highest value of polygalacturonase activity was 0.59 ± 0.01 U g-1 IDS reported at day 6 of cultivation.

Trichoderma viride, cellulase activity, endoglucanase; exoglucanase, β-glucosidase, pectinolytic enzymes, polygalacturonase

Published by University of Zagreb, Faculty of Agriculture, Zagreb, CROATIA
Sponsored by Ministry Science and Education of the Republic of Croatia