The present study aims to examine the chemical composition of the essential oils of the leaves of Garcinia nigrolineata. The essential oil was extracted by hydrodistillation and characterized by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). In total, 37 chemical components were identified in the essential oil which made up 98.3 % of the total oil composition. The essential oil is composed mainly of β-caryophyllene (25.2 %), α-humulene (12.8 %), valencene (6.2 %), α-cadinol (5.8 %), and germacrene D (5.5 %). To the best of the authors’ knowledge, this is the initial study that has looked into the essential oil composition of Garcinia nigrolineata collected from Malaysia.