Agriculturae Conspectus Scientificus, Vol 75, No 4 (2010)

Mould Growth and Aflatoxin Accumulation Affected by Newly Synthesized Derivative of Coumarine Treatment of Maize Hybrid

Lejla DURAKOVIĆ, Mihaela BLAŽINKOV, Branka ŠEOL, Alma TUDIĆ, Frane DELAŠ, Marijan BOŠNJAK, Sanja SIKORA, Andrea SKELIN, Katarina HUIĆ-BABIĆ, Zijad DURAKOVIĆ

Pages: 169-174

Summary


The effect of newly synthesized derivative of 11-hydroxy-7-imino-7H-7a, 12-diazabenzo/α/anthracen-6-one, on growth and aflatoxin B1 and G1 (AFB1 and AFG1) accumulation by toxigenic mould Aspergillus fl avus ATCC 26949 was studied on a solid substrate (maize grains) to determine the possible use of this compound as a mean of controlling aflatoxin accumulation. Experiments were carried out in a stationary culture at temperature of 28oC during 21 days. The growth of mould was monitored by measuring the analysis of chitin as glucosamine, as a criterion, and concentration of AFB1 and AFG1 was measured by HPLC method using Hewlett-Packard instrument with fluorescence detector. Concentration of investigated coumarine of 0.05 mmol x g-1 stimulated mould growth and aflatoxin accumulation, but concentration of 0.2 mmol x g-1 or higher produced an inhibitory effect. In the presence of 0.2 and 0.5 mmol x g-1 of this compound, mould growth was decreased by 22% and 65%, respectively. Concentration of AFB1 in these investigations was reduced by 30% and 90%, and concentration of AFG1 was reduced almost completely in respect to values obtained in control experiments. In experiments with 2.0 mmol x g-1 of this inhibitor no synthesis of both investigated aflatoxins was found in the sample although small increment of biomass was detected.

Keywords


moulds; chitin; aflatoxins; antifungal agents; coumarine

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