The effect of feeding palmitic acid-enriched protected fat (PPF) supplement at two levels to increase energy density of diets was tested. In experiment 1, 21 multiparous lactating Holstein cows were fed on a basal diet without PPF supplementation (Control) or supplemented with 250 g (MG250) or 500 g PPF (MG500) for 13 weeks. In experiment 2, 12 adult Barki sheep were fed a basal diet without PAF supplementation (Control), or supplemented with 25 g (ME25), or 50 g of PPF (ME50 treatment) for 1 month. In experiment 1, MG250 treatment increased (P