Introducing resistant hybrids as a low-cost and environment-friendly strategy is an effective approach to disease management. The first step in developing resistant hybrids is to identify pollinator lines that are resistant as the paternal parent. The study aimed to diagnose Rhizoctonia rot disease resistance in 47 lines derived from five different populations of pollinators. The lines were evaluated for their resistance to Rhizoctonia under mini-plot conditions. Rhizoctonia solani AG-2-2 isolate was used to artificially infect the roots. The number of plants and roots were counted, and disease and harvest indexes were calculated to assess the resistance rate using the selection index of ideal genotype (SIIG) method. To compare with the SIIG output, biplot and cluster analysis statistical techniques were utilized to validate the results. Population P.107 and P.121 illustrated desirable potential resistance to Rhizoctonia across various pollinator populations. Based on SIIG criterion, the pollinator lines No.19 (S1-980022), No.3 (S1-980004), No.1 (S1-980002), No.20 (S1-980025) and No.25 (S1-980032) were identified as the most resistant lines, which was in accordance with the f indings from cluster and biplot analysis. In essence, these pollinator lines were introduced as resistant paternal parents to provide resistant hybrids for future breeding programs.