Agriculturae Conspectus Scientificus, Vol 89, No 1 (2024)

Pseudomonas plecoglossicida Based Silver Nanoparticles: Characterization, Effect on Growth and Antioxidative Enzymes on Pisum sativum L.

Sivaranjani GOPALAKRISHNAN, Abitha BENSON, Somanathan THIRUNAVUKKARASU, Manoharan Melvin JOE, Suresh DHANARAJ

Pages: 17-25


The current work focuses on the synthesis of silver nanoparticles utilizing rhizobacteria and screening for their effectiveness in terms of plant growth promotion and antioxidative defence ability in inoculated Pisum sativum L. plants. Pseudomonas plecoglossicida Nishimori, et al. (2000), isolated from Kundrathur region of Chennai, Tamil Nadu, India, was used for the synthesis of silver nanaoparticles. The synthesized silver nanoparticle was characterized by X-Ray diffraction analysis (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Dynamic Light Scattering (DLS) and Field Emission Scanning Electron Microscopy (FE-SEM) analysis. The efficacy of the plant growth-promoting bacteria synthesized nanoparticle was assessed in terms of growth and antioxidant activity in Pisum sativum plants. P. plecoglossicida exhibited Indole-3-acetic acid (IAA) production of 7.8 mg/l, P solubilization index of 35 and a high Hydrogen Cyanide (HCN) production. Silver nanoparticles synthesized by WRR55 were validated by peak values of 28.04, 32.45 and 46.44 by XRD analysis. The FTIR spectra revealed the features of the C-O extension, the C-H extension of alkenes and the NH extension of amides. DLS indicated that the particle is nanoscale based on the peak that was obtained between 10 and 100 nm. The produced silver nanoparticles have a spherical shape and a particle size between 30 and 50 nm, according to Field emission scanning electron microscopy (FE-SEM) analysis. When compared to the control, silver nanoparticles produced by WRRS5 demonstrated superior germination and plant growth. At 60 ppm concentration, biosynthesized particles demonstrated increased activity in superoxide dismutase (SOD) activity of 24.2 U mg Protein-1, ascorbate peroxidase (APX) activity of 1.90 mg Protein-1, and Catalase (CAT) activity of 297.4 U mg Protein-1.


Pseudomonas plecoglossicida, silver nanoparticle, nano-biofertilizer, FE-SEM, PGPR

Full Text: PDF