In present study, C18 (determinate) and C19 (indeterminate) cucumber lines were crossed and the hybrid plants were self-pollinated to produce the F2:3 generation. Thirteen ISSR (Inter Simple Sequence Repeat) primers, two retrotransposons and two IRAP (Inter-Retrotransposon Amplified Polymorphism) combination primers were screened for QTLs (Quantitative Trait Loci) controlling plant height in cucumber. Five QTLs using SIM (Single Interval Mapping) were identified but only two major of them were observed using CIM (Composite Interval Mapping) method for plant height in F2:3 generation. The distance of one of the identified QTLs to nearest marker was very low and 0.01 CentiMurgan (cM), which showed a high linked marker to this QTL.