Nutritive value of food should be an imperative and represents content of amino acids, vitamins, minerals, etc. Macrominerals (P, K, Ca, Mg) have an irreplaceable role in the functioning of living organisms and are part of all important biomolecules. Therefore, the goal of present research was to determine the influence of fertilization on content of macrominerals in red beet. A field trial (2003-2005) was set up in a hilly part of Croatia according to the Latin square method with four types of fertilization (control, 50 t stable manure ha-1, 500 and 1000 kg NPK 5-20-30 ha-1). Results show that the highest red beet phosphorus content was determined in treatment with stable manure. Higher level of applied potassium had depressing effect on calcium and magnesium uptake. By fertilization is possible to raise content of macrominerals in red beet, but it is recommended to apply combination of organic and mineral fertilizers.