Agriculturae Conspectus Scientificus, Vol 72, No 2 (2007)

Genotypic Characterisation of Indigenous Rhizobium leguminosarum bv. viciae Field Population in Croatia

Mihaela BLAŽINKOV, Sanja SIKORA, Darko UHER, Dubravko MAČEŠIĆ, Sulejman REDŽEPOVIĆ

Pages: 153-158


Genetic diversity of thirteen Rhizobium legumnosarum bv. viciae strains isolated from different field sites in continental part of Croatia was investigated. All rhizobial isolates were obtained either from plants grown in pots containing soil samples or from field grown plants. The strains were analyzed for DNA polymorphism using two DNA fingerprinting methods - randomly amplified polymorphic DNA (RAPD-PCR) and repetitive extragenomic palindromic- PCR (rep-PCR). Both methods resulted in very similar grouping of strains. Cluster analysis of rep- and RAPD-PCR profi les showed significant differences among Rh. leguminosarum bv. viciae isolates. The highest differences were detected among reference strains and all field isolates revealing considerable genetic diversity of rhizobial field populations. These results suggest the presence of adapted indigenous Rhizobium leguminosarum bv. viciae strains, probably with higher competitive ability, whose symbiotic properties have to be evaluated in further investigations.


field pea; indigenous strains; RAPD; rep-PCR; Rhizobium leguminosarum bv. viciae; symbiotic nitrogen fixation

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