Determination of soil temperature regimes is important to establish the diagnostic horizons that are used for classification of soils. Temperature regimes are determined according to Soil Taxonomy, and soils are allocated into six regimes. From the agricultural aspect, Croatia is divided into three regions: Pannonian, Mountainous and Adriatic and into nine sub-regions. The present analysis shows the average annual cycle of soil temperatures in the period 1981 - 2010 at depths of 10 and 50 cm. According to the average annual soil temperatures, the soils in Middle and South sub-region of the Adriatic region belong to the category of soils with the Thermic temperature regime, and the soils in other seven sub-regions are classified as soils with the Mesic temperature regime. Furthermore, differences between air temperature and soil temperature have been established. Theory that states that the average annual soil temperature can be roughly determined by adding 1°C to the average annual air temperature, was tested on depths of 10 cm and 50 cm and has not proven to be entirely correct.