In the period from 2005 to 2009 the influence of different tillage methods and timelines on chemical properties of soil during cultivation of maize (Zea mays L.) and soybean (Glycine max) in crop rotation was researched at testing grounds of Križevci College of Agriculture (N: 460 01’ 12’’; E: 160 34’ 28’’). Almost all researched chemical properties of soil indicated more favourable results with the application of spring and autumn primary tillage with reduced number of secondary tillage interventions. The smallest content of total nitrogen and to plant available potassium in soil was in control method of the most intensive tillage and it was determined at the end of the research. The worst results regarding humus quantity were observed in control tillage method. The applied spring primary soil tillage had unfavourable influence on soil reaction. All tillage methods, except the autumn method of primary tillage and secondary tillage with multi-tiller after a four year period indicated less plant available phosphorus. From the perspective of sustainable management, the application of tillage methods with reduced number of secondary tillage interventions, achieved more favourable results.