Wild type virulent Agrobacterium tumefaciens strains viz. AtTp0120, AtTe0121, AtMo0122 and AtMi0123 (accession number was given according to their respective host plants) were identifi ed from four dicot plants viz. Tagetes patula, Tagetes erecta, Moringa oleifera and Mangifera indica, respectively, of Rajshahi University campus, Rajshahi, Bangladesh. Isolated strains were confirmed as A. tumefaciens on the basis of their morphological, physiological and biochemical features, antibiotic sensitivity, phytopathogenicity tests, and agarose gel analysis of plasmid DNA in comparing with type strian of A. tumefaciens (ATCC23308T). Supported by statistical analysis of the number of induced tumors in potato disc strain AtTp0120 from Tagetes patula was identified as more virulent than the other isolates.

Agrobacterium tumefaciens; wild type; virulence; phytopathogenicity; antibiotic sensitivity and plasmid DNA

Published by University of Zagreb, Faculty of Agriculture, Zagreb, CROATIA
Sponsored by Ministry Science and Education of the Republic of Croatia