The aim of the present study was to evaluate the antioxidant, anti-inflammatory and antibacterial activities of Pavetta siamica Bremek leaves from the Rubiaceae family. The study also aimed to identify the phytochemical components responsible for these bioactivities. The result revealed that n-hexane extract showed variable activity against all tested bacteria which are Staphylococcus aureus (14.7 ± 0.4 mm), Enterobacter aerogenes (9.0 ± 1.0 mm), Enterococcus faecalis (10.0 ± 1.0 mm) and Pseudomonas aeruginosa (8.3 ± 0.4 mm). The antioxidant activity of all extracts showed significant differences between less polar and more polar extracts with total phenolic content calculated as gallic acid equivalents (mg·L-1), ranged from 704 ± 38.0 to 12480 ± 75.3 mg, whereas the radical scavenging activity was evaluated at 517 nm with a value ranging between 3.71% ± 0.13 to 90.11% ± 0.05. On the other hand, the anti-inflammatory activity of less polar extracts showed 100% inhibition at a constant dosage of 500 μg'mL-1 in both anti-denaturation and anti-hyaluronidase assays. Phytochemical screening of extracts revealed the presence of flavonoids, phenols and tannins in both methanol and aqueous extracts while glycosides, terpenoids and steroids were found in less polar extracts.