The aim of this study was to optimize the rooting of regenerated shoots from nodal segments of three genotypes of fig named: ‘Bargchenari’, ‘Dehdez’ and ‘Runu’. For that purpose, the bottom of shoots (about 5 mm) was dipped in Indole-3-butyric acid (IBA) solution of different concentrations: 500, 1000, 1500, 2000 mg L^-1 and control for 5 seconds and then cultured in sand medium. The test was performed in a completely randomized design with at least 5 replications. In ‘Runu’, the highest rooting percentage and the number of roots per shoot (100% and 15.67, respectively) were observed in concentration of 2000 mg L^-1 ‎IBA, whereas, ‘Bargchenari’ and ‘Dehdez’ produced the highest rooting percentage and the root number per shoot at concentrations of 1000 and 1500 mg L^-1 IBA respectively. Three months after transferring rooted shoots to soil mixtures, ‘Bargchenari’ genotype showed the highest survival rate (80%), shoot length (2.5 cm) and leaf number (4.65 leaves per shoot). In relation to survival percentage, the best medium was perlite, but plantlets showed better growth on perlite + peat medium.