Detrimental impact of certain mitogenome mutations on sperm quality traits, and consequently on male fertility is well documented in humans. With a quantitative genetic mixed model, we analysed the impact of cytoplasmic effects, maternal lineages treated as random effect, on sperm quality traits in 554 Austrian Fleckvieh bulls. We have observed that 2% of the phenotypic variance for transformed total number of spermatozoa is due to cytoplasmic (maternal lineage) effects. Regarding percent of viable live spermatozoa, no cytoplasmic effects were detected. However, the observed effects still need to be further evaluated from three perspectives, the analysis of the mitogenome polymorphism effects and the impact of the mitogenome effects on the realised fertility as well as on the whole production economically.