The drosophilid fauna was studied in two integrated production vineyards in the northern part of Croatia (Međimurje County). The flies were sampled using apple vinegar trap during the autumn of 2016. A total of 317 drosophilid individuals belonging to eight species and two genera were collected. Seven species come from the genus Drosophila Fallen 1823, while only one came from the genus Chymomyza Czerny 1903. The most abundant species, with 69% of the total catch, was Drosophila suzukii (Matsamura, 1931) followed by Drosophila simulans Sturtevant, 1919 (19%) and Drosophila melanogaster Meigen, 1830 (6%). Four species were characterized as alien to Europe (Chymomyza amoena (Loew 1862); Drosophila immigrans Sturtevant, 1921; Drosophila melanogaster and Drosophila suzukii). Invasive alien species, D. suzukii, first recorded in Croatia in 2010, has become the dominant species in the investigated area. Results from this research represent the first faunistic insights of the family Drosophilidae in Croatian agroecosystems.